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5A, compared with the control group of non-treated, BSO-treated and NCBSO-treated cells, bright spots gradually appeared on the cell surface after 6 h of NS and NSBSO treatment, and extensive bright spots were observed in B16 cells treated with NSBSO for 9 h

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Mechanistically, these changes appear to coincide with elevated NAD+ and activation of metabolic gene programs consistent with increased energy expenditure

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Pharmaceutical grade NAD+ (Nicotinomide adenine dinucleotide) 20%

Cells were incubated with the ROS indicator, DCFH-DA, a membrane-permeable compound that becomes fluorescent upon intracellular esterase-mediated hydrolysis, yielding DCFH