The RRLuc-His sequence was then amplified using primers 20 and 21 and inserted into the NcoI and KpnI sites of pIVEX1.3_eYFP-His to generate pIVEX1.3_RRLuc-His
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Bravo-Gutierrez OA, Falfan-Valencia R, Ramirez-Venegas A, Sansores RH, Ponciano-Rodriguez G, Perez-Rubio G
Amid dust motes lit by the daylight that slanted through chinks in the walls, I tried to paint a picture: of workers, often women, standing in the open-air tying shed adjacent to the barn, pulling cotton twine from a spool to skillfully string the three aligned stems, or hands, of leaves to a one-inch- square, six-foot-long tobacco stick