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First, cerebrolysin inhibits GSK3beta, directly reducing tau phosphorylation, one of the two hallmark pathologies of Alzheimer disease (alongside amyloid-beta accumulation)

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The cells were lysed in lysis buffer [10 mM Tris (pH 7.4), 10 mM KCl, 2 mM MgCl, 1 mM EGTA, 1 mM DTT, 0.05% (v/v) Triton X-100 and 1 protease inhibitor (Pierce)] for 30 min on ice

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