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Dealing with Precipitation Issues What it looks like: visible particles or haze forming after the peptide seemed dissolved, usually a pH or solvent mismatch

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Grove, Melanocortinergic Activation by Melanotan II Inhibits Feeding and Increases Uncoupling Protein 1 Messenger Ribonucleic Acid in the Developing Rat, Endocrinology, Volume 148, Issue 7, 1 July 2007, Pages 32793287, [3] Ct I, Sakarya Y, Kirichenko N, Morgan D, Carter CS, Tmer N, Scarpace PJ

After overnight cold precipitation of the samples and a long cold centrifugation at 14000 x g, the supernatants were discarded, and the pellets washed with acetone