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These cell lines were cultured in RPMI1640 medium (Gibco cat#11875119) supplemented with 5% Fetal Bovine Serum (BioWest, cat#S1620), 1% Penicillin/Streptomycin (Fischer Scientific, cat#15-140-122) and 1ug/ml Puromycin (Gibco, cat#A1113802)

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To view a copy of this licence, visit About this article Cite this article Hu, X., Jiang, L., Yuan, H

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Remarkably, studies in neonatal immune cells and young animals demonstrate that these FAAs can have a wide range of effects on cells in developing intestines, also at concentrations similar to their levels in human milk

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