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Boris M, Goldblatt A, Galanko J, James SJ (2004) Association of MTHFR gene variants with autism

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Briefly, tissues were homogenized in 500 L of RNAiso plus, with total RNA separated by mixing with 100 L of chloroform, and isolated by isopropanol precipitation

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Additionally, inflammatory signals lead to increased expression of adhesive molecules on ECs, including vascular cell adhesion molecule 1 (VCAM-1), intercellular adhesion molecule 1 (ICAM-1), and E-selectin