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Separation of GSH and GSSG was achieved by means of a gradient elution at a constant flow rate of 0.65 ml min 1 , the injected sample volume was 10 l

Similarly, glutathione peroxidase 4 (GPX4), an essential mitochondrial antioxidant enzyme protecting cells from lipid peroxidation and ferroptosis [126], and nuclear factor erythroid 2-related factor 2 (NFE2L2), which governs the oxidative stress response and mitochondrial homeostasis [127, 128], initially increase but then markedly decrease in later disease stages [115]

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Finally, the relaxitivity of the sample that shows the best performance has been determined