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The islets were collected with a cell strainer (70 M) and recovered in RPMI cultivation media supplemented with 10% FBS, 100 U/ml penicillin/streptomycin, 10 mM HEPES pH 7.4, 2 mM Gibco GlutaMAX (Thermo Fisher, 35050061), 1 mM Gibco Sodium Pyruvate (Thermo Fisher, 11360-039)