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ROS and oxidatively modified macromolecules function as damage-associated molecular patterns (DAMPs), engaging pattern recognition receptors (PRRs) on microglia, including Toll-like receptors (TLRs) and NOD-like receptors (NLRs), to initiate inflammatory cascades ( Microglia, the resident immune cells of the CNS, are tightly regulated under physiological conditions to maintain neural homeostasis

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During the entire experiment, the mice were housed under rigorously controlled conditions, including a temperature of 23C 2C, a relative humidity of 45% 5%, and a consistent 12-hour light/dark cycle