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parapsilosis W23 cultures (1x10 7 cells/ml) was first added to a black glass-bottomed 96-well plate (Grenier) followed by pre-warmed RPMI 1640 in the ratio 1:10 and incubated shaking for 24 h at 20 rpm (37C)
Either way, it goes to work faststimulating the hypothalamus , the part of the brain responsible for arousal and motivation
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