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Subsequently, added 60 L of 50% acetonitrile, vortexed with shaking at 1700 rpm for 10 minutes at 8 C, centrifuged at 15,000 g for 20 minutes at 4 C, and aspirated the supernatant and stored at 80 C until analysis

doi: 10.1016/j.cell.2013.11.030 163 BeggsSSalterMW

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Serious but rare side effects: Acute pancreatitis (severe abdominal pain) Gallstones Allergic reactions (rash, swelling, difficulty breathing) Monitor for signs of dehydration due to gastrointestinal side effects