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The separation gradient was run with 2% to 5% B over 1 minute, 5% to 10% B over 9 minutes, 10% to 20% B over for 27 minutes, 20% to 35% B over 10 minutes, 35% B to 99% B over 10 minutes, a 2 minute hold at 99% B, and finally 99% B to 2% B held at 2% B for 5 minutes 17

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However, due to its sulfur content, a very strong, sulfuric or rotten egg smell is a tell-tale sign of significant degradation

It does not substitute for professional medical, dermatological, or healthcare advice

Sealed containers with fresh desiccant offer superior humidity protection compared to original vial packaging alone

doi: 10.1093/hmg/ddy261 103 WangFLiuYNiFJinJWuYHuangYet al