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Freezing a reconstituted peptide solution causes ice crystal formation, which can disrupt the peptide's tertiary structure through mechanical shear stress

Sekhar et al., 2011b)

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As a final test for the physiological significance of the 6-AH family blockade of Met-dependent cellular outcomes, we evaluated the ability of D-Nle-Cys-Ile-NH-(CH 2 )5-CONH 2 to inhibit the formation of pulmonary colonies by B16-F10 cells after tail vein injection in mice

While the treatment with Ru(II)-OH or NP2 slightly elevated the ROS levels inside the hypoxic cancer cells, the treatment with NP4 showed a strong enhancement of ROS