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Western Blot Analysis Cell extracts were prepared in lysis buffer containing Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 2 mM DTT, 2 mM PMSF and 1% Triton X-100 followed by ultrasonication method

Discussion In this study, 12 weeks of taurine supplementation significantly improved insulin sensitivity and lowered serum glucose and serum lipid concentrations in diabetic OLETF rats that were 50 weeks old at the beginning of supplementation

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You must expect some level of swelling, numbing and bruising since it is a surgery after all