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High pH fractionation- TMT labeled, combined, and dried samples were resuspended in 300 L of Buffer A (5% v/v MeCN, 95% v/v H 2 O, 0.1% v/v formic acid) and fractionated by centrifugation using a peptide desalting column (Pierce, #89852)

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10.1016/j.ajhg.2019.10.009 347 WalkerA

Yang, H., et al., Tumour necrosis factor alpha induces co-ordinated activation of rat GSH synthetic enzymes via nuclear factor kappaB and activator protein-1