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In brief, HEK293T cells (American Type Culture Collection (ATCC), CRL-3216) were cultured in complete DMEM (Gibco, 11965-092) supplemented with 10% (v/v) FBS (VWR, 97068-085) and 1% penicillinstreptomycin (VWR, 16777-164) at 37 C under 5% CO 2

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However, a 2025 HSS Journal systematic review (544 articles screened, 36 included 35 preclinical, 1 clinical) concluded that high-quality clinical evidence remains limited and use should be approached with caution