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Lactobacillus acidophilus suppresses non-alcoholic fatty liver disease-associated hepatocellular carcinoma through producing valeric acid

To create the tnaA mutant, the DNA fragment containing the kanamycin resistance cassette for homologous recombination was amplified by PCR using the primers F- tnaA -FRT and R- tnaA -FRT and the plasmid pKD4 as the template

Step 2: Start with a clean slate

Leave it on for 15 minutes and rinse off with water

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Instructions: Heat shrimp + veggies in pan 5 min