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Preparation of stock solution of n-acetyl l-cysteine 10 mg of n-acetyl l-cysteine was accurately weighed, transferred into a 10-ml volumetric flask and dissolved using a small amount of 0.1 N KOH, and then, the volume was made up using distilled water

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Huffman DM, Farias Quipildor G, Mao K, Zhang X, Wan J, Apontes P, Cohen P, Barzilai N

Total RNA was extracted using the TRIzol method, and the extracted RNA was reverse-transcribed into cDNA using the HiScript II Q RT SuperMix for qPCR (+ gDNA wiper) kit (Vazyme, China)

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