doi: 10.1681/ASN.V1482084 12 WannerCTonelliMKidney Disease: Improving Global Outcomes Lipid Guideline Development Work Group Members
These associations were slightly more robust among the women who continued taking the medications during at least some of the pregnancy
Regulation of vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 in human vascular smooth muscle cells
Acneiform rash induced by EGFR inhibitors: review of the literature and new insights
2.4 Western blot analyses Whole cell protein lysates were prepared by lysis in radio-immunoprecipitation assay buffer (150 mM NaCl, 2 % NP-40, 0.5 % sodium deoxycholate, 0.1 % SDS, 50 mM Tris pH 8.0, 1 mM PMSF, 1 g/ml aproptinin, 1 g/ml pepstatin, 1 g/ml leupeptin, 1 mM NaF, 1 mM sodium orthovanadate, 0.1 mM sodium molybdate, 10 mM sodium pyrophosphate) at a density of 4 10 7 cells/ml on ice, followed by addition of equal volumes of Laemmli protein sample buffer (60 mM TrisCl pH 6.8, 2 % SDS, 10 % glycerol, 5 % -mercaptoethanol, 0.01 % bromophenol blue) and heated to 95 C for 5 min prior to separation on SDS-PAGE gels and transfer to 0.45 m nitrocellulose membranes
MFN2 deficiency impairs mitochondrial transport and downregulates motor protein expression in human spinal motor neurons