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Western blotting Antibodies used in cellular studies include anti-p-p70 S6K (Thr389) (1A5) (mouse mAb, 9206, 1:1,000 for western blotting), anti-p70 S6K (rabbit, 9202, 1:1,000 for western blotting), anti-4E-BP1 (rabbit, 9644, 1:1,000 for western blotting), anti-p-4E-BP1 (Ser65) (rabbit, 9456, 1:1,000 for western blotting) and anti-RAPTOR (24C12) (rabbit, 2280, 1:1,000 for western blotting) from Cell Signaling Technology

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In step-a), Gln(Trt)-AIa-AIa-Lys(C18diacidmono-t-butyI-y-Glu(AEEA-AEEA)-OtBu)-Glu (OtBu)-Phe-IIe-AIa-Trp(Boc)-Leu-VaI-Arg(pbf)-Gly-Arg(pbf)-Gly-OtBu (Fragment-6) was condensed with Fmoc-Ser(tBu)-Tyr(tBu)-Leu-Glu(OtBu)-Gly-OH (Fragment-3) in presence of coupling agent to obtain Fmoc-Ser(tBu)-Tyr(tBu)-Leu-Glu(OtBu)-Gly-Gln(Trt)-AIa-AIa-Lys (C18diacidmono-t-butyI-Y-Glu(AEEA-AEEA)-OtBu)-Glu(OtBu)-Phe-IIe-AIa-Trp(Boc)-Leu- Val-Arg(pbf)-Gly-Arg (pbf)-Gly-OtBu in in-situ manner

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