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Protein concentrations in lysates were determined by Protein Assay Dye Reagent (BioRad #5000006), diluted in 2x Laemmli sample buffer (BioRad Cat#1610737) with fresh -mercaptoethanol (BioRad #1610710), and heated at 95C for 5 minutes

Implications of oxidative stress and homocysteine in the pathophysiology of essential hypertension

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J Allergy Clin Immunol 119:13031310

main effect of post-exposure days, F 4,24 = 16.513, P 0.05)