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The effect of GHK and GHK-PEG treatment on SH-SY5Y cell viability was assessed by plating cells in 96 well plates at 5000 cells per well and incubated for 24 hrs

Green flags signs your protein intake is adequate: Energy levels are stable across the day, without significant mid-afternoon crashes Strength feels maintained you can do what you could do before you started the medication Hair loss, if present, is slowing or has stabilised (some shedding is normal in the first two to three months) Your weight is falling gradually and your body shape is visibly changing alongside it Nails are growing normally and not breaking or thinning excessively Red flags signs your protein intake may be insufficient: Persistent fatigue that doesn't improve with rest and isn't explained by poor sleep - this may be semaglutide-related fatigue worth understanding Noticeable weakness or strength loss things that were easy feel harder Rapid weight loss (more than 11.5 kg per week) that feels disproportionate to how you're eating Significant hair shedding (beyond what is expected in the first 12 weeks) Slow wound healing or recurring infections None of these are emergency symptoms, but they are feedback worth acting on

OSCs can drive cancer cell proliferation and accelerate the onset of CRC (Kroschwald et al., 2018)

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Unregulated use in gyms remains a concern and how we navigate this in SA will be interesting

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