NanoBiT assays were carried out as previously described (28): 24 hours after transfection, cells were incubated in 3 mM glucose for a minimum of 2 hours, detached and resuspended in NanoGlo Live cell substrate (N2012, Promega), 1:20 dilution in HBSS, and seeded onto an opaque white 96-well half-area plate
Upon reaching adulthood, worms were subsequently moved to freshly-seeded 60 mm NGM plates every 24 h
Tremor and a racing heartbeat are how most people notice blood sugar dropping, and a beta-1 blocker suppresses exactly those signals
MeAgain's medication graph shows an estimated level across the 7-day cycle, so the week has shape instead of guesswork
No live mouse was censored
Regular consultations ensure that any adverse effects are addressed promptly and medication doses are adjusted as needed for safety and effectiveness