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The cells were divided into the following five groups: Normoxia, H/R, H/R + semaglutide (5 mmol/l), H/R + semaglutide (5 mmol/l) + rapamycin (autophagy inducer), and H/R + semaglutide (5 mmol/l) + 3-MA (autophagy inhibitor)

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Hyperammonemic encephalopathy, sometimes fatal, has been reported following initiation of valproate therapy in patients with urea cycle disorders, a group of uncommon genetic abnormalities, particularly ornithine transcarbamylase deficiency