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RNA isolation and quantitative reverse transcription-polymerase chain reaction Total RNA was isolated using the ISOGEN reagent (Nippon Gene) and reverse transcribed to cDNA using the ReverTra Ace qPCR RT Kit (Toyobo, Osaka, Japan)

PLoS Journals , Scientific Reports , vol

On the other hand, those with severe kidney problems taking high amounts of B12 may see a high level of toxicity since the excess vitamin B12 wont get eliminated from the body easily

Complex I deficiency in Parkinsons disease frontal cortex

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