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After 1 hour (T1), 2 L of choline chloride (Sigma Aldrich, C7017) (50 M) was added, and the samples were incubated for 3 (T3) or 12 h (T12) from the start of the experiment (T0)

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Every opening and piercing is an opportunity for contamination, even despite the preservative, the principle of minimal handling applies

For example, dietary LC (150 mg/kg diet) enhanced the rates and amounts of lymphatic absorption of -tocopherol and fat in ovariectomized rats [133] and increased liver -tocopherol and lowers liver and plasma triglycerides in aging ovariectomized rats [134]

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Moize VL, Pi-Sunyer X, Mochari H, et al