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Primary hepatocytes on Day 2 were stimulated by H 2 O 2 (30% hydrogen peroxide, 7722-84-1) with a concentration gradient of 0, 10, 50, 100, 200 nM for 24 h, or a time gradient of 0, 1, 6, 12, 24 h, or pretreated with 100 ng/ml IGF-1 (Insulin-like growth factor-1, PeproTech, 081701)

Centrifugation was then performed at 5000 g for 10 min

What is the main function of glutathione in the body

The presence of high concentrations of oxygen and iron in red blood cells exposes them constantly to oxidative stress as reactive oxygen species (ROS) are formed

A 2020 review details their mechanisms, pharmacology and clinical trials in indications ranging from GH deficiency diagnosis to visceral adiposity and GI dysmotility