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To ensure complete inhibition, incubate the sample 15 min at 37 C for thrombin or 30 min at 25C for factor Xa (see Note 32)

Within the retromer complex, SNX proteins are primarily responsible for membrane remodeling and are thus essential for the vesicle budding and formation

If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids

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Dung dch hon nguyn cha 150 mg/mL glutathione