Cell lysate was centrifuged and the soluble roGFP proteins purified via a Ni 2+ loaded HiTrap Chelating HP Column (GE Healthcare, www.gelifesciences.com)
Guo J, Shen Y, Lin X, Chen H, Liu J

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Expression and distribution of glucagon-like peptide1 receptor mRNA, protein and binding in the male nonhuman primate (Macaca mulatta) brain
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