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Since polyphenols can act as PAINS in biochemical assays (autofluorescence, redox cycling, aggregation, metal chelation), in-vitro hits without orthogonal validation were downgraded

They describe this as both surprising and welcome

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Mass spectrometric determination of genistein tissue distribution in diet-exposed SpragueDawley rats

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2.6 Human umbilical vein endothelial cells and tube formation assay Human umbilical vein endothelial cells (HUVECs) were purchased from Lonza (Walkersville, MD USA) and cultured in Endothelial Cell Basal Medium-2 (EBM-2