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Purified recombinant ERK5 was mixed and incubated with serially diluted compounds in a 384-well plate for 10 min at 25 C

Research demonstrates that IGF-1 LR3 activates the same downstream pathways as native IGF-1, primarily the PI3K/Akt/mTOR and Ras/MAPK/ERK cascades, but with greater potency due to its prolonged receptor availability