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Insulin: a growth hormone and potential oncogene

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For immunoprecipitation, cell lysates were prepared as before and rotated 1 mg proteins with antibody at 4 C overnight, then immunoprecipitated by Protein A/G beads

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125 This protective mechanism is likely facilitated through the activation of the adenosine 5-monophosphateactivated protein kinase (AMPK) pathway, which plays a crucial role in managing cellular stress and the DNA repair process