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Cells were seeded in 96-well plates at a density of 35,000 cells per well and transiently transfected using Lipofectamine 2000 according to the manufacturers protocol

For individuals with type 2 diabetes, this means demonstrating stable treatment and absence of significant diabetes-related complications

The most commonly reported side effects are gastrointestinal, nausea, vomiting, diarrhea, or constipation, especially early in treatment or after a dose increase

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But Ive seen one in eight Americans have been on them

We cant extrapolate from two or three cases to predict what will happen in every pregnancy