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Using SEQRS ( in vitro selection, high-throughput sequencing of RNA, and sequence specificity landscapes), analysis of RNA-binding preference of FBF-2 PUF domain bound to a 150-amino-acid LST-1 fragment containing one of FBF-binding sites revealed a distinct RNA-binding specificity of the FBF-2/LST-1 complex (Qiu et al., 2019)