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Hum Reprod Update (2008) 14(3):24358

The treadmill training protocol was performed for 1 h daily on an EXER-4 treadmill (Columbus Instruments, Columbus, OH, United States) as described before ( At the end of the training period, the animals were sacrificed, and the liver and kidneys were quickly excised from the animals and stored immediately at 80C until further analysis (within 1 week)

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In amikacin-treated groups, biofilms were grown in the presence of 418 g/ml amikacin dissolved in TSB