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The mammalian expression vector along with the lentivirus packaging vector (psPAX2) and envelope vector (pMD2.G) were co-transfected into Lenti-X 293 T using X-tremeGENE HP DNA transfection reagent (Roche)

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Proper storage conditions ensure stability and maintain biological activity throughout multi-phase research studies, supporting consistent data collection

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Based on these results, this study proposed an alternative plan for an agricultural community garden to enhance community engagement