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Normalised counts of organoid samples were filtered for the 51 ferroptosis-related genes (Supplementary Table 1) and a hierarchically clustered heatmap was generated depicting gene z-score

[30] showed that particular cytokines found in plasma of ALS patients predict shorter (IL-1 and IL-12) or longer (IL-10) disease duration and suggested that a lesser degree of inflammation might be associated with longer disease duration

Third, while bulk proteomics provides a comprehensive view of cellular differentiation pattern, more precise methods such as single-cell technologies (transcriptomics or proteomics) 38,39 and spatiotemporal single-cell tracking 40,41 would further elucidate the detailed cellular composition and 3D organization during iHLO development

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