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In the fifth step of the mechanism, which is the third chemical step, Tyr9 deprotonates the glutathione thiol, restoring the active site of the enzyme to the initial state

C.FlorquinS.HoutkooperR

This process helps to maintain the delicate balance between antioxidants and free radicals in our bodies, preventing oxidative stress and protecting our cells from damage

After treatment with LBNP, LBNP-PC and LBNP-PC@M for 48 h or different concentrations of LBNP-PC@M for 48 h, 10 L of CCK-8 solution was added to each well and incubated for 1 h at 37 C

[1] Sinha R, Sinha I, Calcagnotto A, et al

High resolution mass spectrometry of GSH (50 mM) in the presence of Ru(II)-OH (2 mM) were directly conducted after preparation and after incubation for 12 h