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For the cell apoptosis experiment, the ARP1-SRD5A1-KD and H929-SRD5A1-KD cells post 2 g/mL treatment for 48 h at a density of 2 10 5 cells were collected and stained using Annexin-V-Allophycocyanin (Annexin-V-APC) (#640941, BioLegend, San Diego, USA) and PI for 15 min in dark conditions, and the apoptosis of the cells was detected using flow cytometer

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Titration experiments were carried out with receptor concentrations in the nanomolar range and increasing concentrations in labeled ghrelin

GLP-1 medications are designed to be taken once every seven days

Biochimica et Biophysica Acta (BBA) 1869(6):166724