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After sedimentation the supernatant was discarded and fresh ice-cold PBS was added to resuspend the intestinal pieces

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We have solved the structures of the pi class GST hP1-1 in complex with its substrate, glutathione, a transition-state complex, the Meisenheimer complex, and an inhibitor, S-(rho-bromobenzyl)-glutathione, and refined them to resolutions of 1.8 A, 2.0 A and 1.9 A, respectively

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The latter may help switch on another enzyme family called protein kinase C (PKC), which is posited to interact with protein synthesis related to hGH synthesis