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Cell Culture and in vitro Model of Hemorrhagic Toxicity Astrocytes and 293T cells (Chinese Academy of Sciences, Shanghai) were cultured in dulbeccos modified eagle medium (DMEM) (Gibco, UA) added 10% fetal bovine serum (Gibco, Australia), following which they were incubated at 100% humidity in an environment containing 5% CO 2

Regarding the form of vitamin B12 used, cyanocobalamin was the most common (n = 14), followed by methylcobalamin (n = 6), hydroxocobalamin (n = 4), and combinations including adenosylcobalamin in isolated cases

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