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The parasite genome demonstrates significant instability characterized by aneuploidy, copy number variations (CNVs), and single nucleotide polymorphisms that collectively facilitate rapid adaptation to therapeutic interventions ( Leishmania to develop resistance through various mechanisms including altered drug transport, target modification, and enhanced metabolic detoxification pathways (Ponte-Sucre et al., 2017)

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In primary rat pituitary cell culture, TNF- can induce a rapid increase in intracellular free calcium concentration, and the time course is highly consistent with the increase in prolactin secretion, suggesting that the calcium signaling pathway plays a key downstream effect in the process of inflammatory factors directly acting on prolactin-secreting cells

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