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Then, the cells were washed twice, fixed and permeabilized with the BD Cytofix/Cytoperm Fixation/Permeabilization Kit (BD Biosciences), and stained with mouse monoclonal anti-FLAG M2-FITC antibody (F4049, Sigma Aldrich

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LV-shBDNF- and LV-shSCR-treated rats were injected with BrdU 24 h or 1 week before euthanasia, and neurogenesis was evaluated by immunohistochemistry

Each freeze-thaw cycle sacrifices potency, so prepare aliquots for single use

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Md S, Abdullah ST, Alhakamy NA, Bani-Jaber A, Radhakrishnan AK, Karim S, et al