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The classification has been described in Table 1

The human GCLC expression plasmid was constructed using the Gateway Cloning system (Thermo Fisher Scientific) and the pCMV-SPORT6-GCLC vector containing a human GCLC cDNA clone (Horizon Discovery Catalog #: MHS6278-202759380, Clone ID: 6052162, NCBI GeneBank Accession: BC039894), the Gateway pDONR221 Vector (Thermo Fisher Scientific), and the pLenti-CMV-Neo-DEST (Addgene Plasmid #17392) destination vector

In comparison, the percentages ( Figure 2C ) of the two aforementioned cell populations had opposite biphasic courses

Obstet Gynecol 2006;108:162-168

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