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For protein extraction, the final pellet was resuspended in 200 uL RIPA lysis buffer, prepared as above, shaken at 3000 rpm on the Disrupter Genie (Scientific Industries) for 15 minutes, 4 C, and centrifuged 10 minutes at 1600 x g, 4 C

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REWIND (Researching Cardiovascular Events with a Weekly Incretin in Diabetes) investigated the use of once weekly dulaglutide on cardiovascular events in patients with type 2 diabetes, revealing a significant reduction in the primary composite outcome [15]