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After mincing to small pieces, they were treated with collagenase type V (Sigma-Aldrich, C9263) and trypsinEDTA (Gibco, 25200-056) and suspended in DMEM supplemented with 1% w/w penicillinstreptomycin and 10% FBS
Activated T cells were transduced with concentrated virus in StemMACS and HTCM supplemented with IL-2 was replenished at 24 h after transduction and every other day
The Fenton reaction, in which iron catalyzes the production of reactive oxygen species (ROS) that target polyunsaturated fatty acids (PUFAs) in cell membranes, can cause ferroptosis in endothelial cells
[DOI] [PubMed] [Google Scholar] 131.Oakley A.J., Lo Bello M., Battistoni A., Ricci G., Rossjohn J., Villar H.O., Parker M.W
PMID: 38431521