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The coupling agent used in the present invention can be selected from but not limited to N,N'-dicyclohexylcarbodiimide (DCC), N,N"-diisopropyl carbodiimide (DIC), l-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC.HC1), N,N"-carbonyl diimidazole (CDI), 1 -[bis(dimethylamino)methylene]- 1H- 1 ,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate (HATU), 2-(lH-benzotriazol-l-yl)-l,l,3,3-tetramethyluronium hexafluorophosphate (HBTU), IH-benzotriazolium l-[bis(dimethylamino)methylene]- 5-chloro-hexafluorophosphate( 1 )-3-oxide(HCTU), (benzotriazol- 1 -yloxy)tris (dimethylamino )phosphonium hexafluorophosphate (BOP), benzotriazol- 1-yl- oxytripyrrolidinophosphonium hexafluorophosphate (PyBOP), l-hydroxy-7-azatriazole (HOAt), 1-hydroxy benzotriazole (HOBt),l-hydroxy-lH-l,2,3-triazole-4-carboxylate (HOCt),O-(benzotriazol-l-yl)-N,N,N',N'-tetramethyluronium tetrafluoroborate (TBTU), N- hydroxysuccinamide (HOSu), N-hydroxysulfosuccinimide (Sulfo-NHS), ethyl cyanohydroxyiminoacetate (Oxyma), 7 -Azabenzotriazol- 1 -yloxy)tripyrrolidinophosphonium hexafluorophosphate (PyAOP), N,N,N',N'-Tetramethyl-O-(N-succinimidyl)uronium tetrafluoroborate (TSTU) or mixtures thereof
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Brainderived neurotrophic factor (BDNF), acting downstream of PI3K activation, enhances dendritic spine plasticity and connectivity in the adult cortex
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