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Lack of follow-up care: Some unregulated clinics may not provide follow-up care or monitoring to ensure patients recover safely from the treatments

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To verify separation trends between different groups, supervised orthogonal partial least squares discriminant analysis (OPLS-DA) was used

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PVDF membranes were blocked with 5% skim milk at room temperature for 2 h and then incubated with primary antibodies at 4 C overnight, followed by incubation with secondary horseradish peroxidase-conjugated antibodies (1:1000, Sungene Biotech, China) for 1 h at room temperature

Morning intake provides daytime protection against environmental stressors, while night-time intake supports the body's natural repair and detoxification processes during sleep