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Red blood cells were lysed with 500 l of 1 BD FACS lysing solution (BD, 349202) for 5 min at room temperature, stopped with 3 ml of PBS, and stained with the antibody mixture consisting of Mac1, Gr1, B220, and CD3 for 15 min at 4 C, followed by flow cytometry analysis (BD, Fortessa)
doi: 10.3389/fcell.2021.683609 Received 21 March 2021 Accepted 30 April 2021 Published 09 June 2021 Volume 9 - 2021 Edited by Jiri Novotny, Charles University, Czechia Reviewed by Flavio Maina, Aix-Marseille Universit, France
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