doi: 10.1016/S0923-1811(02)00015-4 13
Both methods are considered "cold-processing" because they aim to inactivate bacteria while keeping the protein intact
High-dose glycine can reduce the therapeutic effect of clozapine and may potentiate sedation when taken with benzodiazepines, opioids, or other CNS depressants
Available at: Araj SK, Brzezik J, Mdra-Gackowska K, Szeleszczuk
After 2 days of expression, cells were harvested in ice-cold PBS and subsequently lysed in AP-MS buffer (10 mM Tris/Cl pH 7.5, 150 mM NaCl, 10% Glycerol, 0.5 mM EDTA, 0.5% Nonidet P40) supplemented with protease inhibitor cocktail (Roche, Rotkreuz, Switzerland) by sonification, followed by centrifugation (20,000 g for 10 min
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